World Congress of Gastroenterology

WCOG 2019


 
IL-2、IL-15 and stem cell factor enhance cytotoxicity of CD3-CD56+CD16+ NK cells against human hepatocellular carcinoma cell lines
QUAN LU 1 HAO CHEN 1 WEIHONG SHA 1 QIYI WANG 1

1- GUANGDONG PROVINCIAL PEOPLES HOSPITAL AND GUANGDONG ACADEMY OF MEDICAL SCIENCES
 
Background/Aims:

Specific-type natural killer cells play a vital role in anti-tumor by regulating the immune system. To investigate the effects of IL-2IL-15 and stem cell factor on the efficiency of expansion, expression of NKG2D and the cytotoxicity of CD3-CD56+CD16+ NK cells against human hepatocellular carcinoma cell lines.

 

 

Materials and Methods:

 

The CD3-CD56+CD16+ NK cells purified by the sorting method of magnetic microbeads activated cells sorting were expanded in the presence of IL-2 or IL-15 or IL-2/IL-15/SCF with or without anti-NKG2D monoclonal antibody. The efficiency of expansion, the expression of NKG2D and the cytotoxicity of CD3-CD56+CD16+ NK cells against HepG2SMMC-7721 were determined using trypan blue staining, flow cytometry and CCK-8 assay, respectively.

Results:

The efficiency of expansion, the expression of NKG2D and the cytotoxicity of CD3-CD56+CD16+ NK cells treated with IL-2/IL-15/ stem cell factor were significantly increased than those in other groups. Most interestingly, the cytotoxic effects of CD3-CD56+CD16+ NK cells on HepG2 cells were significantly reduced by the pretreatment with anti-NKG2D monoclonal antibody. However, the treatment with the same antibody had little influence on the cytotoxicity of CD3-CD56+CD16+ NK cells against SMMC-7721 cells.

Conclusion:

 IL-2/IL-15/stem cell factor enhances the efficiency of expansion, the expression of NKG2D and the cytotoxicity of CD3-CD56+CD16+ NK cells against human hepatocellular carcinoma cell lines. NKG2D plays an important role in mediating the cytotoxicity of NK cells on specific types of tumor cells.

Keywords:

 Killer cells, natural; Liver neoplasms; Receptor, NKG2D