World Congress of Gastroenterology

WCOG 2019


 
COLONIC RNF186 GENE EXPRESSION IN ULCERATIVE COLITIS PATIENTS
NALAN GÜLŞEN ÜNAL 1 CANSU ÇALIŞKAN KURT 1 NEVIN ORUÇ 1 ÇAĞDAŞ AKTAN 2 AYŞEGÜL KOZAK 2 BAŞAK DOĞANAVŞARGIL 3 ÖMER ÖZÜTEMIZ 1

1- EGE UNIVERSITY FACULTY OF MEDICINE DEPARTMENT OF GASTROENTEROLOGY
2- EGE UNIVERSITY FACULTY OF MEDICINE DEPARTMENT OF MEDICAL BIOLOGY
3- EGE UNIVERSITY FACULTY OF MEDICINE DEPARTMENT OF PATHOLOGY
 
Background/Aims:

Ulcerative colitis (UC) is chronic inflammatory bowel disease which is characterized by inflammation and ulceration of the colonic mucosa. Genome-wide association studies have identified susceptible loci for UC, including a gene that is encoding RING finger protein 186 (RNF186). It was shown that RNF186 controls protein homeostasis in colonic epithelia and regulates intestinal inflammation. However, it is unclear how a role of RNF 186 expression plays for the extention of colitis. The aim of this study is to investigate the expression of RNF 186 in colonic segments biopsies of treatment-naiv UC patients and to compare it healthy individuals.

Materials and Methods:

 This is a prospective study. A total 25 UC patients and 21 healthy indivudials were enrolled in this study between January 2017-January 2019. UC diagnosed with clinical, endoscopical and histopathological findings according to ECCO guidelines. Colonoscopic activity and extention of colitis were noted.  Biopsies from six colonic segments (cecum, ascending colon, transverse colon, descending colon, sigmoid colon and rectum) were evaluated for histopathological changes and RNF186 gene expression. The expression levels of RNF 186 were determined by using template cDNA samples and  qPCR Array study “All-in-OneTM qPCR Mix” (Genecopoeia). Primers / probes specifically designed for our target gene RNF186 and housekeeping gene were used for the study. Final analysis was performed with 2 -ΔΔCT method for the comparison of gene expression.

Results:

A total 25 patients (17 male, 8 female) and 21 healty control were included in the study. Disease extention were E3 in 13 patients, E2 in 10 patients and E1 in 2 patients. RNF186 expression were found significantly decreased in colonic segments of UC patient compared to healthy controls. RNF186 expression in involved colonic segments of UC patients were similar to uninvolved segment of the same patients. RNF186 expression was decreased in involved segments up to 7,15 fold compared to healty controls while it was decreased in uninvolved segments up to 8,44 fold compared to healty controls. RNF186 gene expression rates of involved colonic segments and uninvolved colonic segments were shown in table 1 and table 2, respectively.  

Conclusion:

 This is the first study evaluating the RNF186 gene expression in all colonic segments of treatment-naiv UC patients and healty controls. RNF186 expression were significantly decreased in involved and uninvolved colonic segments in UC patients compared to healty controls. This study revealed that UC is pancolonic genetic disorder mainly orchestrazied RNF186. RNF186 has a protective role and decreased RNF186 gene expression both involved and uninvolved segments in UC patients may suggest its probable role in colonic involvement and progression of disease.

Keywords:

 RNF186, ulcerative colitis, gene expression