World Congress of Gastroenterology

WCOG 2019


 
Bifidobacterium adolescentis ATCC15703 ameliorate chronic colitis by promoting Th2/Treg response and gut microbiota remodeling
LINA FAN 1 SHUJIE CHEN 1 JIANMIN SI 2

1- DEPARTMENT OF GASTROENTEROLOGY, SIR RUN RUN SHAW HOSPITAL, ZHEJIANG UNIVERSITY SCHOOL OF MEDICINE
2- INSTITUTE OF GASTROENTEROLOGY, ZHEJIANG UNIVERSITY
 
Background/Aims:

 Probiotic bacteria have been described to have positive influence on gut health, especially, Bifidobacterium strain play an important role on Gastroenterology homeostasis. In this study, we aim to explore whether B.adolescentis have an impact on prompting protective T cell response in dextran sulfate sodium (DSS) induced chronic colitis.

Materials and Methods:

 C57BL/6 male mice were administrated 3% DSS treatment for 5 days, followed by regular water for 7days. The process was repeated for 3 cycles. At the same time, B.adolescentis ATCC15703 oral gavage with the amount of 1×109CFU was applied daily, When the DSS treatment suspended. At terminal point, blood immune cells and colon lamina propria were isolated for flow cytometry. T cell related cytokine profiling were tested by Mouse Th1/Th2/Treg Array Kit. Inflammation cytokine MCP-1 and IL-6 were detected by qpcr. Tight junction proteins were explored by western blot and qpcr. At last, 16srRNA sequencing was processed on an Illumina MiSeq platform.

Results:

 B.adolescentis gavage ameliorated the DSS induced chronic colitis as evidenced by the significantly decreased diarrhea score, spleen weight and increased colon length. Accordingly, inflammation damage, inflammation infiltration, crypt damage and their cumulative histological grading were decreased in B.adolescentis administration group. In the DSS model, colon lamina propria of B.adolescentis group consisted of more foxp+CD4+T cells and gata3+CD4+T cells, which inhibited the extreme gut inflammation. Similarly, the same phenomenon was observed in blood immune cell. T cell Transcription factor foxp3, gata3 and socs3 mRNA level increased in B.adolescentis group, as well. The distinct effects were found with decreased inflammation cytokine such as MCP-1 and IL-6 and increased TH2 cytokine IL-4/IL-5. In addition, Tight junction protein claudin and occludin enhanced after B.adolescentis applied. 16srRNA sequencing showed B:F ratio evidently increased in B.adolescentis group. In addition, in genus level, Akkermansia and Escherichia-Shigella growth were significantly inhibited.

Conclusion:

B.adolescentis refined the DSS induced chronic colitis by stimulating protective TH2/Treg response, inflammation cytokine arrest and gut microbiota remodeling. B.adolescentis regularly treatment might improve therapeutic effect for inflammation bowel disease. 

Keywords:

 Microbiota, Inflammation bowel disease, Immune response